Related Experiment Video
Updated: May 20, 2026
08:34
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
Tissue augmentation by white blood cell-containing platelet-rich plasma
1Department of Plastic Reconstructive and Aesthetic Surgery, Kijunkai, Yoshikawa Hospital, Sakyo-ku, Kyoto, Japan. smnp99024@nike.eonet.ne.jp
Cell Transplantation
|July 17, 2012
Summary
Enhanced platelet-rich plasma (W-PRP) with white blood cells improved tissue augmentation in mice. Adding bFGF further boosted results, suggesting W-PRP
Area of Science:
- Regenerative Medicine
- Biotechnology
- Dermatology
Background:
- Platelet-rich plasma (PRP) aids wound healing via cytokine release.
- PRP is autologous, minimizing allergic reactions and infection risk.
- Optimized centrifugation can enhance PRP's white blood cell component (W-PRP).
Purpose of the Study:
- To evaluate the tissue augmentation potential of W-PRP compared to standard PRP.
- To investigate the synergistic effect of W-PRP with basic fibroblast growth factor (bFGF).
- To explore W-PRP's application in cosmetic skin rejuvenation.
Main Methods:
- Optimized centrifugal separation to obtain W-PRP from plasma.
- Subcutaneous injection of PRP, W-PRP, and W-PRP+bFGF into the auricles of nude mice.
- Histological analysis, including counting α-smooth muscle actin-positive cells.
Main Results:
- W-PRP demonstrated superior tissue augmentation compared to PRP in mice.
- Co-administration of W-PRP with bFGF resulted in further enhanced tissue augmentation.
- A significant increase in α-smooth muscle actin-positive cells was observed with W-PRP+bFGF treatment.
Conclusions:
- W-PRP shows enhanced tissue augmentation capabilities compared to standard PRP.
- The addition of bFGF potentiates the regenerative effects of W-PRP.
- W-PRP represents a novel cell transplantation concept for tissue regeneration and may benefit cosmetic surgery for skin rejuvenation.
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