Related Experiment Video
Updated: May 20, 2026
15:27
Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
Analysis of cell viability using time-dependent increase in fluorescence intensity
H K Hamalainen-Laanaya1, M S Orloff
1Division of Solid Organ Transplantation, Department of Surgery, University of Rochester Medical Center, Rochester, NY 14623, USA. heli_hamalainenlaanaya@urmc.rochester.edu
Analytical Biochemistry
|July 17, 2012
Summary
This study presents a rapid and cost-effective AlamarBlue assay for assessing primary cell viability. The method accurately measures cell metabolic activity and reduction potential, suitable for high-throughput applications.
Area of Science:
- Cell Biology
- Biotechnology
Background:
- Resazurin (AlamarBlue) dye reduction is a common cell viability assay.
- Current methods are impractical for primary cells due to slow metabolic rates.
Purpose of the Study:
- To develop a robust and rapid AlamarBlue assay for primary cell viability.
- To enable accurate monitoring of cell metabolic activity and reduction potential.
Main Methods:
- Utilized AlamarBlue dye with human mononuclear cells.
- Monitored fluorescence intensity increase over several hours.
- Assayed cell growth rates, cytotoxicity, and sample quality.
Main Results:
- Demonstrated a linear increase in cellular reduction capacity with AlamarBlue.
- The assay proved effective for primary cells, overcoming limitations of slower metabolic activity.
- Showcased applicability for assessing frozen cells and pooled donor samples.
Conclusions:
- The AlamarBlue assay provides a simple, rapid, and cost-effective method for primary cell viability assessment.
- This technique is well-suited for high-throughput studies and immunological research.
- The assay can be adapted to monitor reduction potential in various living cells.
