Related Experiment Videos
Subcellular localization of yeast CDC46 varies with the cell cycle
K M Hennessy1, C D Clark, D Botstein
1Department of Genetics, Stanford University, California 94305.
Genes & Development
|December 1, 1990
Summary
The Saccharomyces cerevisiae CDC46 protein accumulates in the nucleus during interphase. This cell cycle-regulated protein rapidly disappears from the nucleus at the G1-S boundary, preceding DNA replication.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- The cell cycle involves precise regulation of protein localization and function.
- DNA replication initiation is a critical event that must occur only once per cell cycle.
Purpose of the Study:
- To investigate the cell cycle-dependent localization and regulation of the Saccharomyces cerevisiae CDC46 protein.
- To understand the role of CDC46 protein dynamics in cell division and DNA replication.
Main Methods:
- Microscopy to observe CDC46 protein localization in Saccharomyces cerevisiae.
- Cell cycle analysis to correlate CDC46 protein disappearance with specific cell cycle phases.
- Biochemical assays to assess total CDC46 protein levels throughout the cell cycle.
Main Results:
- CDC46 protein accumulates in the nucleus of nondividing yeast cells.
- A rapid decrease in nuclear CDC46 protein occurs upon commitment to cell division.
- This disappearance is not due to changes in total protein levels and coincides with the G1-S transition.
- CDC46 protein is essential for DNA replication.
Conclusions:
- CDC46 protein localization is tightly regulated in a cell cycle-dependent manner.
- Nuclear import of CDC46 occurs post-mitosis, and its rapid removal signals the initiation of DNA replication.
- Protein partitioning provides a mechanism for ensuring once-per-cycle events like DNA initiation.