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Tubularized urethral replacement using tissue-engineered peritoneum-like tissue in a rabbit model.

Gang-Li Gu1, Shu-Jie Xia, Jie Zhang

  • 1Department of Urology, Qilu Hospital, Shandong University, Jinan, China.

Urologia Internationalis
|July 17, 2012
PubMed
Summary

Autologous mesothelial cells show promise for tissue-engineered urethral reconstruction. Seeding cells onto bladder acellular matrices prevented stricture formation in rabbits, creating a neourethra indistinguishable from native tissue.

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Area of Science:

  • Regenerative Medicine
  • Urology
  • Tissue Engineering

Background:

  • Mesothelial cells' potential as seed cells for engineered tissues is under investigation.
  • Tissue-engineered peritoneum-like tissue is explored for urethral reconstruction.
  • Rabbit models are utilized to assess novel reconstructive techniques.

Purpose of the Study:

  • To evaluate mesothelial cells as seed cells for constructing tissue-engineered peritoneum-like tissue.
  • To assess the efficacy of these engineered tissues in urethral reconstruction.
  • To compare outcomes with and without cell seeding in a rabbit model.

Main Methods:

  • Bladder acellular matrices were prepared and seeded with in vitro expanded autologous mesothelial cells.
  • Urethroplasty was performed in rabbits using tubularized matrices, with and without cell seeding (control).

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  • Postoperative assessment included serial urethrography and histological analysis of the neourethra.

    • Main Results:

    • Cell-seeded matrices resulted in wide urethral caliber without stricture formation.
    • Control group animals (no cells) all developed urethral strictures.
    • Histological analysis showed neourethras with organized urothelium and smooth muscle, resembling native tissue over time.

    Conclusions:

    • Autologous mesothelial cells are effective seed cells for urethral reconstruction.
    • Tissue-engineered matrices seeded with mesothelial cells promote successful urethral regeneration.
    • This approach offers a promising alternative for treating urethral defects.