Related Experiment Video
Updated: May 20, 2026
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Published on: January 8, 2020
[Construction and characterization of aroA deletion mutant of Pasteurella multocida strain C51-17]
Dongchun Guo1, Yan Lu, Jiasen Liu
1State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China. gdongchun@hvri.ac.cn
Objective:
We constructed Pasteurella multocida strain C51-17 aroA mutant and characterized its virulence.
Methods:
Suicide recombinant plasmid of pBC-aroA-Tn903 was constructed with kanamycin gene and transformed into Pasteurella multocida by electroporation. aroA mutant strain of P. multocida was screened by kanamycin resistance and chloromycetin sensitivity and identified by PCR. Virulence of aroA mutant strain was tested in mice by intraperitoneal injection.
Results:
The aroA mutant strain of P. multocida C51-17 was successfully constructed. Growth rate of aroA mutant strain was slower within the first 6 hour and similar to the wild-type strain in vitro. The virulence test indicated that aroA mutant strain was significant attenuated and no death was found in mice even inoculated at a dosage of 1.0 x 10(6) CFU.
Conclusion:
The virulence of aroA mutant strain of P. multocida was highly attenuated in mice. The results of this study will contribute to a better understanding of pathogenesis of P. multocida.
More Related Videos
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
05:06Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024