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Updated: May 20, 2026
Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay
Published on: April 3, 2009
Gold nanoparticles induce apoptosis in MCF-7 human breast cancer cells
Manar E Selim1, Awatif A Hendi
1Zoology Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia. manares1@hotmail.com
Background:
Gold nanoparticles have recently been investigated with respect to biocompatibility according to their interactions with cells. The purpose of this study was to examine cytotoxicity and apoptosis induction by well-characterized gold nanoparticles in human breast epithelial MCF-7 cells.
Methods:
Apoptosis was assessed by TUNEL, cytotoxicity by MTT assay and caspase 3, 9, p53, Bax and Bcl expression by real-time PCR assays.
Results:
Gold nanoparticles at up to 200 μg/mL for 24 hours exerted concentration-dependent cytotoxicity and significant upregulation of mRNA expression of p53, bax, caspase-3 and caspase-9, whereas expression of anti- apoptotic bcl-2 was down-regulated.
Conclusion:
To the best of our knowledge this is the first report showing that gold nanoparticles induce apoptosis in MCF-7 cells via p53, bax/bcl-2 and caspase pathways.
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