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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

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Related Experiment Video

Updated: May 20, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

Hematopoietic stem cell development requires transient Wnt/β-catenin activity.

Cristina Ruiz-Herguido1, Jordi Guiu, Teresa D'Altri

  • 1Program in Cancer Research, Institut Hospital del Mar d'Investigacions Mèdiques, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Spain.

The Journal of Experimental Medicine
|July 18, 2012
PubMed
Summary

Wnt/β-catenin signaling is essential for generating hematopoietic stem cells (HSCs) from embryonic endothelial cells in the aorta-gonad-mesonephros region. This pathway is crucial for HSC emergence but not their long-term maintenance.

More Related Videos

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Area of Science:

  • Developmental biology
  • Stem cell biology
  • Regenerative medicine

Background:

  • Hematopoietic stem cells (HSCs) are vital for blood formation and regenerative medicine.
  • HSCs originate from endothelial cells in the aorta-gonad-mesonephros (AGM) region during embryonic development.
  • Understanding HSC generation is key for in vitro HSC production.

Purpose of the Study:

  • To investigate the role of Wnt/β-catenin signaling in HSC emergence from embryonic endothelial precursors.
  • To determine the necessity of β-catenin activity in the aorta-gonad-mesonephros region for HSC generation.
  • To explore the potential of targeting Wnt/β-catenin for in vitro HSC production.

Main Methods:

  • Analysis of β-catenin localization and activity in embryonic mouse aorta.
  • Genetic deletion of β-catenin in embryonic endothelium (VE-cadherin-Cre) and hematopoietic cells (Vav1-Cre).
  • In vitro differentiation of hematopoietic cells from AGM endothelial precursors.

Main Results:

  • Nuclear β-catenin was detected in endothelial cells associated with early hematopoietic clusters in the embryonic aorta.
  • Transient Wnt/β-catenin activity in the AGM region is required for generating long-term HSCs and in vitro hematopoietic cells.
  • Genetic deletion of β-catenin in embryonic endothelium blocked HSC emergence but not adult endothelium contribution.
  • Deletion in hematopoietic cells did not affect HSC emergence.

Conclusions:

  • Wnt/β-catenin signaling is indispensable for the emergence of HSCs from embryonic endothelial cells.
  • This signaling pathway is required for HSC generation in the aorta-gonad-mesonephros region.
  • Wnt/β-catenin activity is necessary for HSC emergence, not for their subsequent maintenance.