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Related Experiment Video

Updated: Feb 15, 2026

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

Engineered intestinal crypt geometry uncovers YAP1-dependent fetal-to-adult transition.

Martti Maimets¹, Mikhail Nikolaev², Cecilia Lövkvist¹

¹Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.

|February 13, 2026

Summary

This summary is machine-generated.

Engineered scaffolds guide fetal intestinal cell development into mature tissues. Cell crowding reduces YAP1 activation, linking tissue geometry to cell fate and offering insights for regenerative medicine.

Keywords:

bioengineering intestine maturation state transitions stem cells

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Last Updated: Feb 15, 2026

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

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Published on: November 15, 2019

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

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Published on: February 6, 2021

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Published on: April 7, 2023

Area of Science:

Developmental biology
Tissue engineering
Stem cell biology

Background:

Intestinal morphogenesis involves complex structural remodeling from a simple tube to a crypt-villus architecture.
The link between structural changes and epithelial cell maturation during intestinal development is not well understood.

Purpose of the Study:

To establish a platform for guiding intestinal morphogenesis and differentiation using engineered scaffolds.
To investigate the role of cell crowding and YAP1 signaling in intestinal tissue maturation.
To uncover geometry-dependent mechanisms linking tissue architecture to cell fate transitions.

Main Methods:

Utilized engineered scaffolds mimicking crypt-like geometries to culture fetal intestinal cells.
Investigated the role of cell crowding and YAP1 signaling pathway.
Modulated YAP signaling in engineered tissues and developing mouse intestines.

Main Results:

Developed a platform for creating mature engineered intestinal tissues that mimic in vivo counterparts.
Demonstrated that cell crowding drives tissue maturation by reducing YAP1 activation.
Showed that modulating YAP signaling impacts epithelial lineage specification in both engineered and developing intestinal tissues.

Conclusions:

Tissue architecture, specifically geometry, influences cell fate transitions during intestinal development.
Cell crowding-induced YAP1 inhibition is a key mechanism linking structure to cell maturation.
This work provides a model for studying intestinal development and informs strategies for stem cell differentiation and regenerative therapies for intestinal disorders.