Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cardiac alpha-crystallin. II. Intracellular localization.

S Longoni1, S Lattonen, G Bullock

  • 1Department Pharmaceutical Research, Ciba-Geigy, Basel, Switzerland.

Molecular and Cellular Biochemistry
|September 21, 1990
PubMed
Summary

A novel cardiac crystallin protein, homologous to alpha-B-crystallin, forms large globular structures in rat hearts. This protein appears to be involved in organizing cytoskeletal filaments within the Z-lines of heart muscle cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Where's your head at? Perceptions and utilisation of concussion prevention and rehabilitation strategies in women's and men's rugby union.

Journal of science and medicine in sport·2025
Same author

Prioritising patient and public involvement and engagement in military settings is vital for effective musculoskeletal injury mitigation programmes.

BMJ military health·2024
Same author

An integrated approach to estimate how much urban afforestation can contribute to move towards carbon neutrality.

The Science of the total environment·2022
Same author

Clinical, metabolic, and molecular genetic characterization of hereditary methemoglobinemia caused by cytochrome b<sub>5</sub> reductase deficiency in 30 dogs.

Scientific reports·2020
Same author

Intra and extra oral clinical manifestations of Rendu-Osler-Weber syndrome: case report and literature review.

Journal of biological regulators and homeostatic agents·2019
Same author

Low-level laser therapy protocols in dental movement acceleration and in pain management during orthodontic treatment.

Journal of biological regulators and homeostatic agents·2019

Area of Science:

  • Biochemistry
  • Cell Biology
  • Structural Biology

Background:

  • The soluble fraction of rat heart contains a major homopolymer component.
  • This component is a polymer of a small protein highly homologous to alpha-B-crystallin.

Purpose of the Study:

  • To isolate and characterize this cardiac protein and determine its function.
  • To investigate the structural organization and cellular localization of the cardiac crystallin.

Main Methods:

  • Isolation of the native cardiac protein and visualization using electron microscopy (negative staining, rotary shadowing).
  • Production of polyclonal antibodies against the cardiac crystallin.
  • Immunocytochemical localization in rat heart myocytes and myofibril preparations.

Related Experiment Videos

Main Results:

  • Electron microscopy revealed homogeneous globular particles (14-16 nM diameter) with torus-like structures.
  • Immunocytochemistry showed a distinct striation pattern in myocytes, localized to the I-band's central region, similar to desmin.
  • Cardiac crystallin was not associated with F-actin but was a contaminant in desmin preparations.

Conclusions:

  • Cardiac crystallin forms large macromolecular complexes in the heart.
  • The protein is localized to the I-band region of myocytes, suggesting a role in cytoskeletal organization.
  • Cardiac crystallin is likely involved in the organization of cytoskeletal filaments, particularly within the Z-lines.