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Related Experiment Videos

Calcitonin exon sequences influence alternative RNA processing.

G J Cote1, I N Nguyen, S M Berget

  • 1Department of Medicine, Veterans Administration Medical Center, Houston, Texas.

Molecular Endocrinology (Baltimore, Md.)
|November 1, 1990
PubMed
Summary
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Tissue-specific RNA processing of calcitonin (CT) pre-mRNA involves a key element in exon 4 that prevents splicing to the calcitonin gene-related peptide (CGRP) exon. This element requires a nearby CT-specific 3' splice site for function.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Calcitonin (CT) and calcitonin gene-related peptide (CGRP) are derived from a single pre-mRNA through alternative splicing.
  • Differential processing dictates tissue-specific expression of CT or CGRP.

Purpose of the Study:

  • To identify cis-acting sequences regulating the tissue-specific splicing of CT/CGRP pre-mRNA.
  • To understand the mechanism preventing splicing to the CGRP exon (exon 5).

Main Methods:

  • Utilized a CT-specific in vitro RNA processing system.
  • Performed deletion mapping of exon 4 sequences.
  • Tested splicing regulation in a heterologous system.

Main Results:

Related Experiment Videos

  • Identified an element within the first 45 nucleotides of CT exon 4 that suppresses splicing to CGRP exon 5.
  • This element functions in a heterologous splicing context.
  • Suppression of splicing to exon 5 requires a proximal CT-specific 3' splice site.

    • Conclusions:

    • A specific exonic element in CT exon 4 is crucial for suppressing alternative splicing to CGRP exon 5.
    • CT-specific splicing relies on the assisted recognition of its 3' splice site by regulatory elements.