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Updated: May 20, 2026

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
Published on: May 13, 2019
[Effect comparison of Wright-Giemsa stain under different conditions and its apply in cell micronucleus test]
Chun-hua Liao1, Huan-feng Bian, Fang Xiao
1Shajing Institute of Inspection, Shenzhen, Guangdong Province, China.
Objective:
To apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.
Methods:
Use Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min).
Results:
After stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.
Conclusions:
Wright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.
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