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Updated: May 20, 2026

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Published on: July 27, 2017
Thin phosphatidylcholine films as background surfaces with further possibilities of functionalization for biomedical
Lara Tauk1, Thierry Thami, Lynda Ferez
1Institut Européen des Membranes, Université Montpellier 2 (ENSCM, UM2, CNRS), Montpellier, France.
Abstract:
Non-specific adsorption is a crucial problem in the biomedical field. To produce surfaces avoiding this phenomenon, we functionalized thin (7-180 nm) poly(methylhydrosiloxane) (PMHS) network films at room temperature (≈20°C) with phospholipids (PL) bearing a phosphorylcholine head. Regardless of their mode of preparation (casting or immersion), all surfaces appeared to be very hydrophilic with a captive air-bubble contact angle stabilized around 40°. The thin films were protein-repellent in phosphate saline buffer pH 7.4 according to analysis by normal scanning confocal fluorescence. Neither was any adsorption or spreading of l-α-phosphatidylcholine liposomes on such films observed. In addition, amino functional groups could be easily attached to the surface remaining available for further functionalization.
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