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Decrease of catecholamine and neuropeptide Y-like immunoreactivity in the glycerol-induced acute renal failure of
J T Cheng1, C L Shen, J J Huang
1Department of Pharmacology, College of Medicine, National Cheng Kung University, Taiwan, Republic of China.
Research in Experimental Medicine. Zeitschrift Fur Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
|January 1, 1990
Summary
Experimental acute renal failure (ARF) in rats significantly reduced kidney levels of noradrenaline (NA) and dopamine (DA). This study investigated catecholamine and neuropeptide Y (NPY) changes in ARF, revealing nerve fiber damage.
Area of Science:
- Nephrology
- Neuroscience
- Biochemistry
Background:
- Acute renal failure (ARF) is a critical condition affecting kidney function.
- Catecholamines and neuropeptide Y (NPY) play roles in renal physiology.
- Understanding neurochemical changes in ARF is crucial for potential therapeutic targets.
Purpose of the Study:
- To investigate the changes in catecholamine (noradrenaline and dopamine) and neuropeptide Y (NPY) levels in experimental acute renal failure (ARF) in rats.
- To assess the integrity of renal adrenergic and dopaminergic innervation during ARF.
Main Methods:
- Experimental ARF was induced in rats using glycerol injection.
- Plasma urea levels and histological examination confirmed ARF.
- Renal concentrations of noradrenaline (NA) and dopamine (DA) were measured using chromatographic analysis with electrochemical detection.
- Renal NPY-like immunoreactivity (NPY-LI) was quantified by radioimmunoassay.
- Immunohistochemistry was used to observe adrenergic nerve fiber degeneration.
Main Results:
- ARF rats exhibited significantly decreased renal levels of NA (from 186.3 to 2.81 ng/g) and DA (from 14.7 to 4.05 ng/g).
- Renal NPY-LI was also markedly reduced in ARF rats (from 435.23 to 4.61 pg/g).
- A parallel decrease in NA and NPY-LI was observed, consistent with degeneration of adrenergic nerve fibers.
Conclusions:
- Experimental ARF leads to substantial damage to both adrenergic and dopaminergic nerve innervations in the kidneys.
- The findings suggest a significant neurochemical disruption in the kidney during ARF.