Related Experiment Video
Updated: May 20, 2026
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
[Effects of mesangial cell on proliferation and function of osteoblast in vitro]
Feng Wu1, Ri-yang Lin, Li-qun He
1Nephrology Department of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China.
Objective:
To explore the effects and mechanism of mesangial cell (MC) on proliferation and function of osteoblast in vitro from growth factors.
Methods:
Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium. MTF was used to checked the proliferation of osteoblast at the end of 24, 48, 72, 120 h; Expression of BGP and OPN were tested 72 h and 144 h after culture; Expression of IGF-alpha and TGF-beta were tested 144 h after culture.
Results:
The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times (P < 0.05); The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group (11.3%, 16.4%, 55.0% and 39.6%); and the level of IGF-alpha and TGF-beta of osteoblast was obviously higher than normal blood serum group (10.1% and 47.7%).
Conclusion:
MC can directly act on osteoblast, promote the proliferation and function of osteoblast by promoting secretion of TGF-beta.
