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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

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Nanofluidic devices towards single DNA molecule sequence mapping

Rodolphe Marie1, Anders Kristensen

  • 1Department of micro- and nanotechnology, Technical University of Denmark, Oersteds plads Building 345east, 2800 Kongens Lyngby, Denmark. Rodolphe.Marie@nanotech.dtu.dk.

Journal of Biophotonics
|July 21, 2012
PubMed

Keywords:
DNAlab-on-a-chipmicrofluidicsnanofluidics

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.