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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: May 20, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

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Published on: July 21, 2023

Artificial DNA and surface plasmon resonance.

Roberta D'Agata¹, Giuseppe Spoto

¹Dipartimento di Scienze Chimiche, Università di Catania, Catania, Italy.

Artificial DNA, PNA & XNA

|July 24, 2012

Summary

Surface plasmon resonance (SPR) biosensors combined with modified oligonucleotides enhance diagnostic capabilities. This review covers 15 years of advances in SPR biosensor technology and molecular recognition studies.

Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Surface plasmon resonance (SPR) biosensors are crucial for molecular recognition studies.
Modified oligonucleotides offer unique properties for biosensor applications.

Purpose of the Study:

To review significant advances in SPR biosensors utilizing modified oligonucleotides over the past 15 years.
To highlight the synergistic benefits of combining SPR technology with novel nucleic acid structures.

Main Methods:

Review of scientific literature focusing on SPR biosensors and modified oligonucleotides.
Analysis of functional and conformational DNA analogs like aptamers and PNA.
Exploration of SPR's role in studying DNAzymes, LNA, and HNA.

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Published on: November 29, 2014

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Last Updated: May 20, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

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Published on: July 21, 2023

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

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Published on: November 29, 2014

Main Results:

Combined use of SPR and modified oligonucleotides significantly expands diagnostic potential.
Functional and conformationally restricted DNA analogs enhance biosensor sensitivity and selectivity.
SPR technology facilitates a deeper understanding of non-natural nucleic acid structures.

Conclusions:

SPR-based biosensors with modified oligonucleotides represent a powerful tool for diagnostics and molecular recognition.
Advances in nucleic acid chemistry and SPR technology continue to drive innovation in biosensing.