Related Experiment Video
Updated: May 20, 2026

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
Published on: June 4, 2021
Technical and practical aspects of (19) F NMR-based screening: toward sensitive high-throughput screening with rapid
Claudio Dalvit1, Anna Vulpetti
1Department of Chemistry, University of Neuchâtel, CH 2000, Neuchâtel, Switzerland. claudio.dalvit@unine.ch
Abstract:
The technical and practical aspects of (19) F NMR-based screening against a macromolecular target are analyzed in detail. A novel method utilizing the relaxation of (19) F homonuclear double quantum coherence is proposed for performing NMR-based binding assays in a direct- or competition-mode format. A combined strategy based on (19) F NMR chemical shift prediction, 2D (19) F NMR DOSY, and 2D (19) F-(1) H NMR long-range COSY experiments is presented for the deconvolution of complex mixtures of fluorinated molecules generated by either addition of single compounds or by chemical synthesis. The approaches presented here allow the screening of complex mixtures, even in the case where the exact composition is not known, and the rapid identification of the binders contained in the mixtures.
Related Concept Videos
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
Two-Dimensional (2D) NMR: Overview
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Applications Of NMR In Biology
The...
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...

