Related Experiment Video
Updated: May 20, 2026

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
[Genetic diversity and volatile components of Asarum sieboldii in Qin-ba region]
Su-ying Hu1, Gang He, Bei Chen
1Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, College of Life Science, Shaanxi Normal University, Xian 710062, China.
Objective:
To analyze the genetic diversity and the volatile components of Asarum sieboldii from seven habitats in Qin-ba region.
Methods:
The genetic diversity of the herb was analyzed by ISSR (inter simple sequence repeat) markers; The relative content volatile components of the herb were dectected by head space solid-phase microextraction gas chromatogrphy-mass spectrometry (HS-SPME-GC-MS). The contents of the 3 main components were analyzed by steam distillation gas chromatography-mass spectrometry (GC-MS).
Results:
57 bands were amplified from 7 populations by 6 reliable primers, 51 of which were polymorphic (89.47% of the total). The cluster analysis presented that these resources were divided into two main groups. There were differences in the chemical components and the contents of Asarum sieboldii from the 6 wild habitats. Except for some same components, many unique components were identified in them respectively. In addition, some components could be detected only in some populations which had smaller genetic distance.
Conclusion:
Cluster analysis shows no direct correlation between genetic distance and geographic distance of Asarum sieboldii in Qin-ba region. The accumulations of some volatile components of Asarum sieboldii are possibly related to genetic diversity.
More Related Videos
Related Concept Videos
Diversity of Archaea IV
Diversity of Archaea III
Diversity of Archaea II
Genetics of Speciation
Diversity of Archaea I
Genetic Variation
Genes exist in different versions called alleles, which...

