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[Fingerprint analysis of Glehniae Radix by TLC]
Xing Su1, Xiang-kun Li, Tao Wu
1Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China. shuxinme111@163.com
Objective:
To establish TLC fingerprint of Glehniae Radix for the identification and quality control of the drug.
Methods:
10 batches of Glehniae Radix commercial drugs collected from different gathering areas and 3 batches from Laiyang, Shandong were used as qualitative identification samples. Falcarindiol, scopoletin umbelliferone and isoimperatorin were used as the chemical reference substances. Double wavelength TLCS was performed with petroleum and ethyl acetate (4:1) as developer,detection wavelength at 300 nm and reference wavelength at 260 nm.
Results:
TLC chromatogram of 13 samples had 8 well-resolved characteristic peaks, in which 4 peaks were falcarindiol, umbelliferone, scopoletin and isoimperatorin, respectively.
Conclusion:
The method is accurate and simple, and can be used for the quality control of Glehniae Radix.
