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NESdb: a database of NES-containing CRM1 cargoes
Darui Xu1, Nick V Grishin, Yuh Min Chook
1Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
Molecular Biology of the Cell
|July 27, 2012
Summary
Researchers created NESdb, a database of nuclear export signals (NESs) that direct proteins out of the cell nucleus. This resource aids in understanding NES function in cellular and disease processes.
Area of Science:
- Molecular Biology
- Cell Biology
- Bioinformatics
Background:
- Nuclear export signals (NESs) are crucial for directing macromolecules out of the cell nucleus.
- NESs are characterized by specific hydrophobic residues that bind to the export receptor CRM1.
- Proteins containing NESs play significant roles in various cellular functions and disease development.
Purpose of the Study:
- To compile a comprehensive database of known NES-containing proteins and their associated export signals.
- To provide a curated resource for researchers studying nuclear export mechanisms.
- To facilitate further investigation into the function and regulation of NES-mediated nuclear export.
Main Methods:
- Manual curation of the published scientific literature to identify NES-containing CRM1 cargoes.
- Compilation of a database (NESdb) containing 221 manually curated entries.
- Annotation of each entry with detailed information on NES and cargo protein sequence and structure, and experimental evidence for NES mapping and CRM1-mediated export.
Main Results:
- Development of NESdb, a manually curated database of 221 NES-containing CRM1 cargoes.
- Each entry in NESdb includes detailed annotations on sequence, structure, and experimental evidence.
- NESdb serves as a valuable resource for the study of nuclear export signals.
Conclusions:
- NESdb provides a centralized and annotated collection of nuclear export signals and their cargoes.
- This database will be regularly updated and will serve as an important resource for the scientific community.
- The resource is freely available to nonprofit organizations, promoting accessibility and further research.
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