Determination of methamphetamine, amphetamine and piperidine in human urine by high-performance liquid chromatography
K Hayakawa1, N Imaizumi, H Ishikura
1Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Abstract:
A high-performance liquid chromatographic method for the determination of trace levels of methamphetamine, amphetamine and piperidine in human urine is reported. The three compounds, extracted into diethyl ether from alkaline urine, were derivatized with dansyl chloride, then separated on a reversed-phase column and detected by chemiluminescence after reaction with bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide. The corresponding peaks obtained from human urine were identified as the dansyl derivatives by mass spectrometry. Methamphetamine levels as low as 2 x 10(-10) M in urine were determined. The sensitivity of the method is higher than that of Simon's reagent test and gas chromatography.
Related Concept Videos
Pulse rhythm
Conversely, an irregular pulse pattern is termed dysrhythmia, stemming from disruptions in cardiac muscle...
Disturbances in Heart Rhythm
Arrhythmias are categorized by their speed, rhythm, and origin. A slow heart...
Holter Monitor: 24-Hour Monitoring
Dysrhythmias IV: Characteristics of Bradyarrhythmias
Dysrhythmias V: Evaluating Dysrhythmias
Dysrhythmias VI: Management of Dysrhythmias


