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More accurate matrix-matched quantification using standard superposition method for herbal medicines.

Ying Liu1, Xiao-Wei Shi, E-Hu Liu

  • 1State Key Laboratory of Natural Medicines-China Pharmaceutical University, Nanjing 210009, China.

Journal of Chromatography. A
|July 28, 2012
PubMed
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This study presents practical methods to overcome matrix effects in herbal medicine analysis. The standard superposition method effectively compensates for signal suppression in quantitative HPLC-MS and HPLC-ELSD analyses.

Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Herbal Medicine Quality Control

Background:

  • Quantitative determination of marker compounds in herbal medicines (HMs) faces challenges due to matrix effects.
  • Blank matrix samples for calibration are typically unavailable for HMs, complicating method validation.
  • Matrix components can interfere with analyte detection, particularly in mass spectrometry (MS) and evaporative light scattering detection (ELSD).

Purpose of the Study:

  • To propose and evaluate practical approaches for minimizing matrix effects in the quantitative analysis of HMs.
  • To assess the impact of matrix effects on high-performance liquid chromatography (HPLC) coupled with MS and ELSD detection for Panax notoginseng saponins.
  • To develop and test matrix-matched calibration methods for accurate HM quantification.

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Main Methods:

  • Developed and tested two matrix-matched methods: standard addition with target-knockout extract and standard superposition with HM extract.
  • Assessed matrix effects on five saponins from Panax notoginseng using HPLC-MS and HPLC-ELSD.
  • Compared the performance of standard superposition method against standard addition for overcoming matrix effects.

Main Results:

  • Matrix components were found to interfere with analyte ionization in HPLC-MS.
  • The standard superposition method proved simple and practical for overcoming matrix effects in HM quantification.
  • Interference with light scattering was observed in HPLC-ELSD but not in UV detection, highlighting detector-specific interference issues.

Conclusions:

  • The standard superposition method is a viable strategy for mitigating matrix effects in quantitative HM analysis.
  • Addressing interference effects is crucial for developing robust quantitative HPLC-ELSD and HPLC-MS methodologies for HM quality control.
  • Detector-specific interference necessitates careful method validation for different analytical techniques used in HM analysis.