Related Experiment Video
Updated: May 22, 2026
08:20
Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
Screening of antithrombotic components from Typhae Pollen by a multidimensional evaluation strategy
Yu-Jia Kuang1, Ze-Bin Weng2, Qian-Qian Chen2
1State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China.
Journal of Ethnopharmacology
|May 20, 2026
Summary
Typhae Pollen (TP) contains flavonoids and organic acids that exhibit significant antithrombotic effects by inhibiting thrombin and platelet aggregation. These compounds offer a scientific basis for TP
Area of Science:
- Phytochemistry
- Pharmacology
- Traditional Chinese Medicine
Background:
- Typhae Pollen (TP) is traditionally used in Chinese medicine for blood circulation and stasis.
- Bioactive constituents and antithrombotic mechanisms of TP are not fully understood.
- This study aims to bridge this knowledge gap.
Purpose of the Study:
- To systematically characterize the chemical constituents of Typhae Pollen (TP).
- To elucidate the antithrombotic activity and underlying mechanisms of TP.
- To provide scientific validation for the traditional use of TP.
Main Methods:
- Phytochemical profiling using UHPLC-QTOF-MS/MS with DFI and NL filtering.
- Multivariate statistical analysis for chemical consistency and regional variation.
- In vitro assays (thrombin inhibition, platelet aggregation) and in vivo zebrafish models.
- Molecular docking and network pharmacology for mechanism elucidation.
Main Results:
- Identified 70 compounds in TP, including flavonoids and organic acids, with good chemical consistency.
- TP extract showed significant thrombin inhibition and reduced ADP-induced platelet aggregation.
- Specific organic acids and flavonoids demonstrated antithrombotic effects in zebrafish models.
- Network pharmacology indicated multiple targets related to inflammation, oxidative stress, and vascular pathways.
Conclusions:
- Flavonoids and organic acids are the primary antithrombotic constituents in Typhae Pollen (TP).
- TP exerts antithrombotic effects through thrombin inhibition and anti-platelet aggregation.
- Findings support the scientific basis for TP's traditional use in managing thrombotic disorders.
