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Influenza

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...

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Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Published on: January 30, 2017

Rapid test for influenza in diagnostics.

Teresa Jackowska¹, Monika Grzelczyk-Wielgorska, Katarzyna Pawlik

¹Department of Pediatrics, Medical Center of Postgraduate Education, Warsaw, Poland. tjackowska@cmkp.edu.pl

Advances in Experimental Medicine and Biology

|July 28, 2012

Summary

During the 2009/2010 season in Poland, rapid influenza diagnostic tests identified A/H1N1v in children. These tests proved valuable for clinical diagnosis when vaccines were unavailable.

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Area of Science:

Pediatrics
Virology
Infectious Diseases

Background:

No specific vaccines for influenza A/H1N1 were available in Poland during the 2009/2010 epidemic season.
Influenza surveillance and diagnosis are critical during epidemic periods, especially for novel strains.

Observation:

During November-December 2009, 17 children with suspected influenza were tested.
Rapid influenza diagnostic tests (QuickVue Influenza A + B) detected influenza type A in 24% of these children.
Seven children were hospitalized, and six were confirmed positive for A/H1N1v by real-time reverse transcription polymerase chain reaction (RT-PCR).

Findings:

The initial clinical presentation of influenza A/H1N1v was unspecific in the observed pediatric cases.
Rapid influenza testing demonstrated high utility in diagnosing influenza A/H1N1v.
RT-PCR confirmed the presence of A/H1N1v RNA in hospitalized children.

Implications:

Rapid influenza diagnostic tests are crucial tools for timely clinical management, particularly in the absence of specific vaccines.
Early and accurate diagnosis aids in appropriate patient care and potentially limits disease spread.
This study highlights the importance of diagnostic capabilities for emerging influenza strains in pediatric populations.