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Development of 12 microsatellite markers for Platycrater arguta (Hydrangeaceae) endemic to East Asia
Xin-Shuai Qi1, Na Yuan, Ying-Xiong Qiu
1Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, Zhejiang University, Hangzhou, People's Republic of China.
Premise Of The Study:
Microsatellite markers were characterized in Platycrater arguta, a rare endemic shrub from eastern China and southern Japan, to investigate its population genetic structure, phylogeographic patterns, and breeding system.
Methods And Results:
Using both the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol and the compound microsatellite marker technique, we developed 12 microsatellite markers. All primers showed polymorphism when assessed in 43 individuals from two populations in eastern China and southern Japan. Overall, the number of alleles ranged from five to 21, with an average of 14.91 alleles per locus.
Conclusions:
These markers can be used in further studies on population genetics, phylogeography, and breeding system of P. arguta.
