Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shaping cell therapy manufacturing with metabolic understanding and control.

Trends in biotechnology·2026
Same author

Ferritin nanoparticles displaying rift valley fever virus glycoprotein elicit potent dendritic cell activation in vitro.

Virology journal·2026
Same author

Bridging process diversity in cell therapies toward standardization of manufacturing workflows.

Cytotherapy·2026
Same author

3D co-cultures of primary human hepatocytes and Kupffer-like cells to address innate immune responses to rAAV.

Scientific reports·2025
Same author

Opioid-free anesthesia in robotic prostatectomy: pain management without transversus abdominis plane block.

Minerva anestesiologica·2025
Same author

Site-directed tyrosinase conjugation on engineered ferritin retains immune recognition.

Scientific reports·2025

Related Experiment Video

Updated: May 20, 2026

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
08:25

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

Three-dimensional HepaRG model as an attractive tool for toxicity testing.

Sofia B Leite1, Iwona Wilk-Zasadna, Jose M Zaldivar

  • 1Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, Portugal.

Toxicological Sciences : an Official Journal of the Society of Toxicology
|July 31, 2012
PubMed
Summary

HepaRG cells in 3D spinner-bioreactors offer a cost-effective hepatic model for drug testing. This system demonstrates long-term liver-like function and aids in assessing drug-induced toxicity and biotransformation.

More Related Videos

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
12:24

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

Published on: September 30, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
08:25

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
12:24

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

Published on: September 30, 2021

Area of Science:

  • Hepatology and Toxicology
  • Bioreactor Technology
  • Drug Metabolism and Pharmacokinetics

Background:

  • Three-dimensional (3D) cell cultures in bioreactors offer advanced models for liver research.
  • HepaRG cells are a validated cell line for in vitro toxicology and drug metabolism studies.
  • Spinner-bioreactors provide a scalable and cost-effective platform for cell culture.

Purpose of the Study:

  • To evaluate HepaRG cells cultured in 3D spinner-bioreactors as a hepatic system for toxicological applications.
  • To assess the biotransformation capacity, including CYP450 enzyme induction and UGT activity.
  • To demonstrate the long-term functionality and liver-like performance of the bioreactor system.

Main Methods:

  • Culture of HepaRG cells in a 3D spinner-bioreactor system.
  • Assay of CYP450 enzyme induction (CYP1A2, 2B6, 2C9, 3A4) and UGT activity.
  • Long-term monitoring of albumin secretion and transporter localization via immunofluorescence.
  • In silico modeling to analyze acetaminophen (APAP) hepatotoxicity data.

Main Results:

  • Demonstrated stable albumin secretion and CYP3A4 induction for 7 weeks.
  • Observed formation of tissue-like structures and polarized transporters.
  • Confirmed bioactivation of APAP and its cytotoxicity in a high-throughput compatible system.
  • Showcased reduced time for cell culture differentiation.

Conclusions:

  • 3D HepaRG cell cultures in spinner-bioreactors provide a valuable, liver-like system for toxicological studies.
  • The system is suitable for routine drug testing and toxicodynamic approaches.
  • This approach enhances understanding of in vitro cell behavior and drug-induced toxicity.