Effect of electromagnetic field on the polymerization of microtubules extracted from rat brain

Microtubules (MTs) are ubiquitous eukaryotic proteinaceous filaments showing a hollow cylindrical structure. MTs are composed of α-β-tubulin heterodimers arranged in linear protofilaments. MTs have a significant electric dipolar moment along their axes, which makes them capable of being aligned parallel to the applied electromagnetic field direction. Tubulin heterodimers were purified from rat brains. MTs were obtained by polymerization in vitro. Samples of microtubules adsorbed under and without electromagnetic fields with 500 Hz frequency. Our results demonstrate the effect of electromagnetic field with 500 Hz frequency to increase the polymerization of MTs. Some relevant patents are also outlined in this article.