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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Capillary electrophoresis-electrospray ionization-mass spectrometry interfaces: fundamental concepts and technical
Grégoire Bonvin1, Julie Schappler, Serge Rudaz
1School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva 4, Switzerland.
Journal of Chromatography. A
|August 1, 2012
Summary
This review details capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) interfaces, crucial for analyte analysis. It covers ESI fundamentals, electrochemical effects, and interface classifications for improved performance.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis (CE) coupled with electrospray ionization (ESI) and mass spectrometry (MS) is a vital analytical technique.
- A significant challenge in CE-ESI-MS is the development of effective interfaces for optimal performance.
Purpose of the Study:
- To review the fundamental aspects of electrospray ionization (ESI) processes.
- To discuss the electrochemical reactions influencing CE-ESI-MS performance.
- To categorize and evaluate current CE-ESI-MS interfaces.
Main Methods:
- Review of fundamental ESI processes: spray formation, droplet evolution, and ion production.
- Detailed discussion of electrochemical reactions in CE and ESI.
- Classification of interfaces based on operating flow rate (electrospray vs. nanospray).
Main Results:
- The review elucidates the three key stages of ESI.
- Electrochemical reactions significantly impact CE-ESI-MS sensitivity and performance.
- Interfaces are classified into electrospray and nanospray regimes, each with distinct characteristics.
Conclusions:
- Effective CE-ESI-MS interfaces are critical for successful analyte analysis.
- Understanding ESI fundamentals and electrochemical effects is essential for interface optimization.
- The review provides a summary of current interfaces, their pros, cons, and applications.
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