A sandwich assay for quantitative detection of transcription factors in cell lysate
Zhiyuan Fang1, Wenjuan Zhang, Chenchen Ge
1Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China.
The Analyst
|August 1, 2012
Abstract:
A double-stranded DNA (dsDNA) mediated sandwich assay was developed for quantitative detection of transcription factors. The detection limit for human recombinant c-jun protein is 2.5 ng, and for c-jun protein the limit is as low as 0.625 μg of cell lysate.
