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Updated: May 20, 2026

Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass
Published on: April 18, 2020
Enzymatic conversion of xylan residues from dilute acid-pretreated corn stover
Joseph Shekiro1, Erik M Kuhn, Michael J Selig
1National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. joseph.shekiro@nrel.gov
Abstract:
Enzymatic conversion of oligomeric xylose and insoluble xylan remaining after effective pretreatment offers significant potential to improve xylan-to-xylose yields while minimizing yields of degredation products and fermentation inhibitors. In this work, a commercial enzyme cocktail is demonstrated to convert up to 70 % of xylo-oligomers found in dilute acid-pretreated hydrolyzate liquor at varying levels of dilution when supplemented with accessory enzymes targeting common side chains. Commercial enzyme cocktails are also shown to convert roughly 80 % of insoluble xylan remaining after effective high-solids, dilute acid pretreatment.
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