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Expression of VEGF and MMP-9 and MRI imaging changes in cerebral glioma
Yang Liu1, Yang Zhou, Xiu-Shi Zhang
1Department of Radiology, The 3rd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081.
Abstract:
The purpose of the present study was to investigate the association of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) expression with the histopathological grading of tumors in cerebral glioma. A total of 45 patients with pathologically confirmed cerebral glioma were divided into two groups: a low-grade group (grades I and II, 21 cases) and a high-grade group (grades III and IV, 24 cases). Immunohistochemical staining of tumor samples showed the percentages of tumors expressing VEGF and MMP-9 in the high-grade group to be 95.83 and 75%, respectively, significantly higher than those of the low-grade group (66.67 and 23.81%, P<0.05 and P<0.01, respectively). The magnetic resonance imaging (MRI) results indicated that the peripheral edema index (EI), enhancement percentage (EP), and the maximum diameter of the tumor in the high-grade group were significantly higher than those in the low-grade group (P<0.05, P<0.01, and P<0.05). Moreover, the expression of VEGF and MMP-9 was positively correlated with EI, EP and the maximum diameter of the tumor (P<0.05). Therefore, VEGF and MMP-9 expression were correlated to the invasion of glioma. The association of their expression levels with EI, EP and the maximum tumor diameter indicates that these markers may be used to estimate tumor malignancy for future clinical diagnosis and treatment.
Insights
Vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) are significantly elevated in high-grade cerebral gliomas. Their increased expression correlates with tumor invasion and imaging markers, aiding malignancy assessment.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Medical Imaging
Background:
- Cerebral glioma grading is crucial for treatment decisions.
- Identifying reliable biomarkers for glioma malignancy is essential.
Purpose of the Study:
- To investigate the association between VEGF and MMP-9 expression and the histopathological grade of cerebral gliomas.
- To explore the correlation of these markers with MRI-derived tumor characteristics.
Main Methods:
- Immunohistochemical staining was used to assess VEGF and MMP-9 expression in 45 glioma samples.
- Patients were categorized into low-grade (I-II) and high-grade (III-IV) groups.
- Correlation with MRI parameters like edema index (EI) and enhancement percentage (EP) was analyzed.
Main Results:
- High-grade gliomas showed significantly higher VEGF and MMP-9 expression compared to low-grade gliomas (P<0.05, P<0.01).
- MRI metrics (EI, EP, tumor diameter) were significantly higher in high-grade gliomas.
- VEGF and MMP-9 expression positively correlated with EI, EP, and tumor diameter (P<0.05).
Conclusions:
- VEGF and MMP-9 expression levels are associated with cerebral glioma invasion and malignancy.
- These markers, along with MRI findings, may assist in estimating tumor grade for clinical diagnosis and treatment planning.
