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Updated: May 20, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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Published on: September 3, 2013

Continuous sorting of microparticles using dielectrophoresis.

D F Chen¹, W H Li, H Du

¹School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.

Journal of Nanoscience and Nanotechnology

|August 2, 2012

Summary

This study introduces a flexible dielectrophoresis (DEP)-based microfluidic device for continuous particle sorting. The system effectively sorts multiple particles in microchannels for biomedical applications.

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Area of Science:

Biomedical Engineering
Microfluidics
Particle Manipulation

Background:

Particle sorting is crucial for biomedical applications but integrating it into microdevices is challenging.
Existing methods often lack flexibility and continuous processing capabilities.

Purpose of the Study:

To develop an effective and flexible dielectrophoresis (DEP)-based microfluidic device for continuous sorting of multiple particles.
To demonstrate the device's capability for precise particle fractionation within a microchannel.

Main Methods:

The device utilizes a DEP focusing unit and a Movable DEP Trap (MDT) formed by microelectrodes and a transparent top electrode.
Particles are focused into a single stream and then sorted by rapidly switching applied voltages to the MDT.
Continuous flow sorting of microparticles was demonstrated.

Main Results:

Successful continuous sorting of microparticles into different fractions was achieved.
The addressable nature of the Movable DEP Trap allowed for dynamic sorting control.
The device demonstrated flexibility in particle manipulation within a microchannel.

Conclusions:

The proposed DEP-based microfluidic sorter offers an effective solution for continuous particle and cell sorting.
This technology has potential applications in sample preparation and cell sorting for analytical processing.
The device's flexibility and addressable trap enhance its utility in complex microfluidic systems.