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Assay of aspartylglycosylaminase by high-performance liquid chromatography
1Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
Analytical Biochemistry
|October 1, 1990
Abstract:
An aspartylglycosylaminase assay based on high-performance liquid chromatographic analysis of the substrate aspartylglucosamine and product aspartate is described. Aspartylglucosamine and aspartate are derivatized with phenylisothiocyanate and resolved by reverse-phase chromatography. The detection limit for the compounds is 2 pmol. The method can be used for analysis of aspartylglycosylaminase activity in crude cell extracts and tissue samples.