Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Influence of A-Site Deficiency and Ca Concentration on the Electrical and Crystallographic Properties of (Nd<sub>0.2</sub>Sr<sub>0.7-</sub> Ca <sub></sub> ) <sub></sub> Ti<sub>0.95</sub>Fe<sub>0.05</sub>O<sub>3-δ</sub>-Based Fuel Electrode for Solid Oxide Cells.

ACS applied energy materials·2024

Same author

The implementation of whole-genome sequencing for <i>Mycobacterium tuberculosis</i> in Vietnam.

IJTLD open·2024

Same author

Isotope effects in dynamics of water isotopologues induced by core ionization at an x-ray free-electron laser.

Structural dynamics (Melville, N.Y.)·2023

Same author

Evaluating the performance of temporal and spatial early warning statistics of algal blooms.

Ecological applications : a publication of the Ecological Society of America·2022

Same author

Ambient pressure x-ray photoelectron spectroscopy setup for synchrotron-based in situ and operando atomic layer deposition research.

The Review of scientific instruments·2022

Same author

Energy Transfer into Molecular Vibrations and Rotations by Recoil in Inner-Shell Photoemission.

Physical review letters·2018

Search research articles

Related Experiment Video

Updated: May 19, 2026

Spatial Separation of Molecular Conformers and Clusters

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

Size selective spectroscopy of Se microclusters.

K Kooser¹, D T Ha, E Itälä

¹Department of Physics, University of Turku, FIN-20014 Turku, Finland. kunkoo@utu.fi

The Journal of Chemical Physics

|August 3, 2012

Summary

This summary is machine-generated.

This study investigates selenium clusters (Se(n)) using advanced spectroscopy. Researchers determined their electronic structure, ionization potentials, and dissociation energies, providing key insights into cluster stability and fragmentation.

More Related Videos

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

Published on: June 20, 2019

Related Experiment Videos

Last Updated: May 19, 2026

Spatial Separation of Molecular Conformers and Clusters

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

Published on: June 20, 2019

Area of Science:

Physical Chemistry
Materials Science
Atomic and Molecular Physics

Background:

Selenium clusters (Se(n)) are crucial in materials science, but their electronic properties and stability are not fully understood.
Investigating small selenium clusters (n ≤ 8) is essential for understanding bulk material behavior.

Purpose of the Study:

To determine the electronic structure and photofragmentation patterns of selenium clusters (Se(n), n ≤ 8).
To experimentally measure ionization potentials and theoretically calculate ionization and dissociation energies.

Main Methods:

Size-selective photoelectron-photoion coincidence spectroscopy using vacuum-ultraviolet synchrotron radiation.
Computational analysis of potential geometrical structures and ionization energies of Se(n) clusters.
Derivation of dissociation energies from experimental ionization energies and calculated enthalpies of formation.

Main Results:

Experimental ionization potentials for Se(n) clusters (n ≤ 8) were successfully extracted.
Calculated ionization energies showed good agreement with experimental data.
Theoretical fragment ion appearance energies and dissociation energies were estimated, providing insights into cluster stability.

Conclusions:

The study provides a comprehensive understanding of the electronic structure and stability of small selenium clusters.
Experimental and theoretical data were combined to elucidate photofragmentation mechanisms.
Findings contribute to the fundamental knowledge of cluster physics and chemistry.