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Virus-poly(3,4-ethylenedioxythiophene) biocomposite films.

Keith C Donavan1, Jessica A Arter, Gregory A Weiss

  • 1Department of Chemistry, University of California, Irvine, California 92697-2025, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|August 4, 2012
PubMed
Summary

Virus-poly(3,4-ethylenedioxythiophene) (virus-PEDOT) biocomposite films efficiently incorporate M13 bacteriophage. Higher virus concentrations in solution lead to increased virus loading in the PEDOT film, impacting its conductivity and topography.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Conductive polymers like poly(3,4-ethylenedioxythiophene) (PEDOT) have diverse applications.
  • Biocomposite materials offer unique properties by integrating biological components with synthetic materials.
  • Bacteriophage M13 is a well-characterized virus suitable for biomaterial functionalization.

Purpose of the Study:

  • To investigate the efficient incorporation of bacteriophage M13 into electropolymerized PEDOT films.
  • To characterize the physical and electrical properties of virus-PEDOT biocomposite films.
  • To determine the relationship between virus concentration in solution and its loading within the PEDOT film.

Main Methods:

  • Electropolymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of M13 bacteriophage.
  • Quartz crystal microbalance (QCM) for direct mass measurement during electrodeposition.
  • Scanning electron microscopy (SEM) and atomic force microscopy (AFM) for topographical analysis.
  • Conductive tip AFM for measuring electrical conductivity perpendicular to the film plane.

Main Results:

  • Virus-PEDOT biocomposite films were successfully prepared with M13 bacteriophage concentrations up to 15 nM.
  • Virus incorporation into the PEDOT film increased linearly with the virus concentration in the electrolyte solution.
  • An efficient incorporation ratio of approximately 450:1 (virus in film to virus in solution) was achieved.
  • Virus incorporation resulted in increased film roughness and a decrease in electrical conductivity, from 270 μS/cm to 50 μS/cm with increasing virus loading.
  • Displayed affinity peptides on the virus surface did not significantly affect incorporation efficiency.

Conclusions:

  • Electropolymerization is an effective method for creating virus-PEDOT biocomposite films with controlled virus loading.
  • The physical and electrical properties of PEDOT films are significantly modulated by the incorporation of M13 bacteriophage.
  • Virus-PEDOT biocomposites show potential for applications requiring tailored conductive biomaterials.