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Updated: May 19, 2026
The Left Pneumonectomy Combined with Monocrotaline or Sugen as a Model of Pulmonary Hypertension in Rats
Published on: March 8, 2019
[Pathophysiological characteristics of rat pulmonary hypertension and cor pulmonale induced by monocrotaline]
Jun-shan Li1, Chao-liang Lng, Wen-yu Cui
1Cardiovascular Drug Research Center, Institute of Health and Envimnental Medicine, Academy of Military Medical Sciences, Beijing, China.
Objective:
To explore the mechanism of pulmonary hypertension and Cor Pulmonale rat models induced by monocrotaline (MCT).
Methods:
Twenty Wistar male rats were randomly divided into normal control group and model group (n= 10), which received a single intraperitoneal injection of MCT solution (50 mg/kg , the first day) or dissolvant, respectively. On day 28 after MCT administration, the hemodynamic parameters were assessed; levels of tumour necrosis factor-alpha (TNF-alpha), nitric oxide (NO), endothelin-1 (ET-1), B-type natriuretic peptide(BNP) in pulmonary tissue or blood were measured using radio immunoassay or nitrate reductase method.
Results:
28 days after MCT injection, compared with control group, right ventricle systolic pressure (RVSP) increased and heart rate(HR), mean arterial pressure (MAP) decreased; Levels of TNF-alpha, NO, ET-1 in pulmonary tissue or blood increased significantly in MCT group.
Conclusion:
The potential mechanism of MCI- induced pulmonary hypertension and Cor Pulmonale rat models associates with increasing TNF-alpha, NO, ET-1 levels in vivo, which results from inflammatory injury of lung tissue and blood vessels induced by MCT.
