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Related Concept Videos

Plant Cell Wall01:07

Plant Cell Wall

Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
Plant Cell Wall02:43

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...

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Related Experiment Video

Updated: May 19, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Whole plant cell wall characterization using solution-state 2D NMR.

Shawn D Mansfield1, Hoon Kim, Fachuang Lu

  • 1Department of Wood Science, University of British Columbia, Vancouver, Canada. shawn.mansfield@ubc.ca

Nature Protocols
|August 7, 2012
PubMed
Summary
This summary is machine-generated.

New nuclear magnetic resonance (NMR) methods allow rapid plant cell wall analysis without deconstruction. This advances understanding of cell wall biosynthesis and aids selection of renewable materials for bio-based economies.

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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
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Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
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Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

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Last Updated: May 19, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

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Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
07:51

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

Published on: June 10, 2017

Area of Science:

  • Plant Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Plant cell walls are complex structures crucial for biomass and bio-based economies.
  • Traditional methods for cell wall analysis require extensive deconstruction and fractionation.
  • Advances in nuclear magnetic resonance (NMR) offer potential for rapid, whole-cell wall characterization.

Purpose of the Study:

  • To present a protocol for rapid, whole-cell wall characterization using 2D NMR.
  • To demonstrate the utility of this NMR approach for understanding cell wall structure and biosynthesis.
  • To facilitate the selection of plant materials for industrial applications in emerging bio-based economies.

Main Methods:

  • Plant tissue preparation and extraction.
  • Development of solubilization strategies for diverse plant materials.
  • 2D NMR acquisition (15 min-5 h) and data integration for compositional analysis.

Main Results:

  • Successful elucidation of lignin subunit composition and interunit linkage distribution.
  • Comprehensive cell wall polysaccharide profiling.
  • Demonstration of NMR whole-cell wall characterization utility compared to traditional degradative methods.

Conclusions:

  • Rapid, whole-cell wall NMR analysis is feasible without prior deconstruction.
  • This method enhances understanding of plant cell wall biology and biosynthesis.
  • The protocol supports efficient screening of plant materials for bio-based industrial applications.