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Updated: May 19, 2026

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
Published on: January 12, 2024
Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies
Gwendoline Thiery1, Ray L Mernaugh, Heping Yan
1Mass Spectrometry Research Center, School of Medicine, Vanderbilt University, Nashville, TN, USA. gthiery@rics.bwh.harvard.edu
Abstract:
Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each coupled to a unique reporter molecule (i.e., a mass tag) it was possible to simultaneously detect multiple antigens within a single tissue sample with high sensitivity and specificity using mass spectrometry. The capability of imaging multiple antigens at the same time is a significant advance that overcomes technical barriers encountered when using present day approaches to develop assays that can simultaneously detect more than a single antigen in the same tissue sample.
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