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In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
Published on: September 8, 2016
Transcription initiation factor IIB involves in Schwann cell differentiation after rat sciatic nerve crush
Jiao Yang1, Jianhua Cao, Youhua Wang
1Department of Immunology, Medical College, Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People's Republic of China.
Journal of Molecular Neuroscience : MN
|August 8, 2012
Summary
Transcription Initiation Factor IIB (TFIIB) increases in rat sciatic nerves after injury, primarily in Schwann cells. This suggests TFIIB is involved in peripheral nerve repair and Schwann cell differentiation.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Transcription Initiation Factor IIB (TFIIB) is crucial for transcription initiation.
- Its role in peripheral nerve injury and repair remains uncharacterized.
Purpose of the Study:
- To investigate the spatiotemporal expression of TFIIB in a rat sciatic nerve crush model.
- To explore the function of TFIIB in Schwann cells during nerve repair.
Main Methods:
- Adult rats underwent acute sciatic nerve crush injury.
- Western blot analysis was used to quantify TFIIB expression.
- Immunohistochemistry identified TFIIB localization in Schwann cells.
- In vitro studies used cAMP to induce Schwann cell differentiation and TFIIB-specific siRNA to assess functional impact.
Main Results:
- TFIIB expression increased post-sciatic nerve crush, peaking at 7 days.
- TFIIB upregulation was mainly observed in Schwann cells, co-localizing with Oct-6.
- TFIIB expression rose during in vitro Schwann cell differentiation induced by cAMP.
- TFIIB knockdown via siRNA impaired cAMP-induced Schwann cell differentiation and P0 expression.
Conclusions:
- Peripheral nerve injury upregulates TFIIB expression in the sciatic nerve.
- TFIIB upregulation is associated with Schwann cell differentiation during nerve repair.
- TFIIB may play a significant role in the molecular mechanisms of peripheral nerve regeneration.
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