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Updated: May 19, 2026
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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Published on: March 7, 2019
A noncomplementation screen for quantitative trait alleles in saccharomyces cerevisiae.
Hyun Seok Kim1, Juyoung Huh, Linda Riles
1Department of Genetics, Washington University, St. Louis, Missouri 63108, USA.
G3 (Bethesda, Md.)
|August 8, 2012
Summary
Quantitative noncomplementation screens help identify genes with large effects on traits like colony color or size in yeast. This method rapidly screens thousands of genes, though small-effect alleles remain challenging to pinpoint.
Area of Science:
- Genetics
- Yeast Biology
- Quantitative Trait Loci (QTL) Analysis
Background:
- Mapping quantitative trait alleles is crucial for understanding complex traits.
- Small-effect alleles are difficult to identify using traditional linkage and linkage disequilibrium methods.
- Quantitative noncomplementation offers a direct approach to test individual genes for quantitative trait alleles.
Purpose of the Study:
- To implement a genome-wide noncomplementation screen in yeast to identify quantitative trait alleles affecting colony color and size.
- To assess the utility of noncomplementation for discovering genes influencing quantitative traits.
- To develop a high-throughput assay for large-scale genetic screening.
Main Methods:
- Utilized the yeast deletion collection for a genome-wide screen.
- Developed a high-throughput robotic plating assay to measure colony color and size.
- Employed quantitative noncomplementation and reciprocal hemizygosity analysis for validation.
Main Results:
- Identified a known allele of CYS4 affecting colony color and a novel allele of CTT1 affecting hydrogen peroxide resistance.
- Screened approximately 4700 genes across nine diverse yeast strains.
- Discovered numerous candidate alleles, but reciprocal hemizygosity revealed many false positives due to background effects or secondary mutations.
Conclusions:
- Noncomplementation is a rapid method for identifying large-effect quantitative trait alleles.
- The screen highlighted challenges in detecting small-effect alleles.
- Yeast deletion collection requires careful validation due to potential genetic artifacts.
Related Concept Videos
00:49Complementation Tests
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
02:46Genetic Screens
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...