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Updated: May 19, 2026

Label-free in situ Imaging of Lignification in Plant Cell Walls
Published on: November 1, 2010
[Distribution of lignin in Chinese fir branches determined by ultraviolet microspectrometer]
Yu-Rong Wang1, Xin-Ting Xing, Hai-Qing Ren
1Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China. yurwang@caf.ac.cn
Abstract:
Distribution of lignin in the cell walls of Chinese fir branches emerged in the spring season were first studied by using ultraviolet microscope based on their cell microstructure observation and lignin qualitative measurement by the lightmicroscope and confocal laser scanning microscope. The results showed that the contents of lignin are inhomogeneously distributed in different micro-areas of the cell walls. The order of lignin concentrations is the cell corner>the middle lamellar>the secondary with the absorbance values of ultraviolet wave of 0.489, 0.307 and 0.278, respectively. The result of quantitative analysis consists with that of qualitative analysis. A new measurement method was proposed to study the distribution of lignin content in wood cell walls in CFhina.

