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Related Experiment Video

Updated: May 19, 2026

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
08:22

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

Retinal nerve fiber layer thickness reproducibility using seven different OCT instruments.

Luisa Pierro1, Marco Gagliardi, Lorenzo Iuliano

  • 1Department of Ophthalmology, Vita-Salute University, San Raffaele Scientific Institute, Milan, Italy. pierro.luisa@hsr.it

Investigative Ophthalmology & Visual Science
|August 9, 2012
PubMed
Summary

Reproducibility of retinal nerve fiber layer (RNFL) thickness measurements varies across optical coherence tomography (OCT) devices. For consistent RNFL monitoring, use the same OCT device and operator to minimize measurement bias.

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Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Biomedical Engineering

Background:

  • Optical coherence tomography (OCT) is crucial for diagnosing and monitoring optic nerve head diseases.
  • Reproducibility of retinal nerve fiber layer (RNFL) thickness measurements is essential for clinical utility.
  • Evaluating different OCT devices is necessary to understand their performance in clinical settings.

Purpose of the Study:

  • To assess the reproducibility of RNFL thickness measurements using multiple spectral-domain OCT (SD-OCT) and time-domain OCT devices.
  • To compare inter- and intraoperator agreement across various OCT instruments.
  • To identify potential biases and errors in RNFL thickness measurements among different OCT devices.

Main Methods:

  • RNFL thickness was measured twice by two masked operators on 38 healthy volunteers using six SD-OCTs and one time-domain OCT.

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  • Inter- and intraoperator reproducibility were assessed using intraclass correlation coefficient (ICC), coefficient of variation (CV), and Bland-Altman analysis.
  • Instrument-to-instrument reproducibility was evaluated using ANOVA, and systemic bias/random error were analyzed via structural equation modeling.

    • Main Results:

    • Mean RNFL average thickness varied between 90.08 μm and 106.51 μm across devices.
    • The Heidelberg Spectralis demonstrated the highest inter- and intraoperator agreement for average RNFL thickness (ICC > 0.92, CV < 1.56%).
    • Significant variability in agreement was observed among instruments, with Optovue showing the worst performance.

    Conclusions:

    • The Heidelberg Spectralis exhibited superior inter- and intraoperator reproducibility for RNFL thickness measurements.
    • Scale bias among OCT instruments can impact RNFL monitoring.
    • Consistent RNFL monitoring is best achieved by using the same operator and the same OCT device.