Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD177 Deficiency Defines a Stable Subtype of Human Neutrophil Granulocytes with Tumor Promoting Activity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Active zone plasticity couples sleep need to presynaptic hypophosphorylation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Loss of cardiomyocyte AKT signaling causes deterioration of lipid metabolism and cellular atrophy.

Metabolism: clinical and experimental·2026
Same author

Oxygen-dependent modulation of the human complement system during acute normobaric hypoxia: a translational plasma proteomics study.

Clinical and experimental medicine·2026
Same author

Formation of tight junction-like structures of zonula occludens 2 in platelet-platelet interaction.

Research and practice in thrombosis and haemostasis·2026
Same author

Cardiac mitochondrial proteome of lean, healthy Ossabaw minipigs with predisposition to metabolic syndrome versus that of Göttingen minipigs.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Exosomes from HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinoma: Selective Cargo Packaging.

Journal of proteome research·2026
Same journal

Proteomic Analysis Identifies ATE1-Dependent Arginylation Dysregulation across Meningioma Grades.

Journal of proteome research·2026
See all related articles

Related Experiment Video

Updated: May 19, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Integral quantification accuracy estimation for reporter ion-based quantitative proteomics (iQuARI).

Marc Vaudel1, Julia M Burkhart, Sonja Radau

  • 1Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany.

Journal of Proteome Research
|August 10, 2012
PubMed
Summary
This summary is machine-generated.

Reporter ion quantification methods like iTRAQ and TMT are popular but suffer from interference. We introduce iQuARI to easily estimate these errors, helping scientists assess workflow reliability and potential interference in complex proteome studies.

More Related Videos

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Related Experiment Videos

Last Updated: May 19, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Quantitative Biology

Background:

  • Reporter ion-based quantification (e.g., iTRAQ, TMT) is widely used for complex proteome comparisons due to multiplexing efficiency and cost-effectiveness.
  • A significant limitation is interference between precursor ions, which compromises quantification accuracy.
  • Recent experimental designs aim to mitigate this interference, but assessing their true benefit remains challenging.

Purpose of the Study:

  • To address the difficulty in evaluating the worth of new reporter ion quantification workflows.
  • To provide a method for inline estimation of error rates in reporter ion quantification.
  • To enable scientists to accurately assess sample- and system-dependent interference.

Main Methods:

  • Introduction of a novel method named iQuARI (Interference Quantification and Reporting Interface).
  • iQuARI computes false discovery rates (FDR) specifically for reporter ion quantification.
  • The method is designed for ease of use, analogous to Target/Decoy FDR calculations in peptide identification.

Main Results:

  • iQuARI allows for straightforward estimation of interference levels in reporter ion quantification experiments.
  • Scientists can now accurately assess the impact of interference on their specific samples and experimental systems.
  • The tool facilitates informed decisions about workflow adoption and potential downstream data processing needs.

Conclusions:

  • iQuARI provides a crucial tool for validating the reliability of reporter ion quantification data.
  • Accurate interference estimation empowers researchers to determine if reporter ion quantification is the optimal choice for their study.
  • The method aids in deciding whether to proceed with data analysis or consider alternative quantification strategies.