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Updated: May 19, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
Dietary nitrate does not enhance running performance in elite cross-country skiers
Oliver Peacock1, Arnt Erik Tjønna, Philip James
1Sport, Health and Exercise Science Research Group, Department for Health, University of Bath, Bath, United Kingdom. O.J.Peacock@bath.ac.uk
Purpose:
The objective of this study is to examine the effects of acute ingestion of dietary nitrate on endurance running performance in highly trained cross-country skiers. Dietary nitrate has been shown to reduce the oxygen cost of submaximal exercise and improve tolerance of high-intensity exercise, but it is not known if this holds true for highly trained endurance athletes.
Methods:
Ten male junior cross-country skiers (V˙O(2max)) ≈ 70 mL·kg·min) each completed two trials in a randomized, double-blind design. Participants ingested potassium nitrate (614-mg nitrate) or a nitrate-free placebo 2.5 h before two 5-min submaximal tests on a treadmill at 10 km·h (≈55% of V˙O(2max)) and 14 km·h (≈75% of V˙O(2max)), followed by a 5-km running time trial on an indoor track.
Results:
Plasma nitrite concentrations were higher after nitrate supplementation (325 ± 95 nmol·L) compared with placebo (143 ± 59 nmol·L, P < 0.001). There was no significant difference in 5-km time-trial performance between nitrate (1005 ± 53 s) and placebo treatments (996 ± 49 s, P = 0.12). The oxygen cost of submaximal running was not significantly different between placebo and nitrate trials at 10 km·h (both 2.84 ± 0.34 L·min) and 14 km·h (3.89 ± 0.39 vs. 3.77 ± 0.62 L·min).
Conclusions:
Acute ingestion of dietary nitrate may not represent an effective strategy for reducing the oxygen cost of submaximal exercise or for enhancing endurance exercise performance in highly trained cross-country skiers.
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