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Updated: May 19, 2026
Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
Stress-response protein expression and DAF-16 translocation were induced in tributyltin-exposed Caenorhabditis
Yun Wang1, Fenglei Jian, Jieyu Wu
1Department of Life Sciences, Huainan Normal University, Huainan 232001, Anhui, China. yunwang2001@163.com
Abstract:
Exposure to tributyltin (TBT) with graded sublethal doses (0, 1, 10, 50 and 200 nM) resulted in the release of reactive oxygen species (ROS) and DNA damage in the nematode Caenorhabditis elegans. After the worms carrying transgenic reporters were exposed to TBT, the expressions of superoxide dismutase (SOD-3), glutathione S-transferase (GST-4) and heat shock proteins (HSP-4, HSP-16.2 and HSP-70) were semi-quantified after exposure. The results indicated that TBT caused dose-dependent induction of SOD-3, GST-4, HSP-4 and HSP-70. Furthermore, TBT exposure also induced DAF-16 translocation from cytoplasm to nucleus. The results implicated that C. elegans might be a potential animal model for TBT level monitoring and toxicity assessment.
