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[Study on the extraction technology and hypoglycemic activity of lectin from Trichosanthes kirilowi]
Qiong Li1, Xiao-Li Ye, Hong Zeng
1College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. qiongli1123@126.com
Objective:
To extract lectins from Trichosanthes kirilowi and study their hypoglycemic activity.
Methods:
The optimal extraction process included the following parameters were conformed by optimization analysis,lectins extracted from Trichosanthes kirilowi was achieved by ammonium sulfate precipitation; The agglutinate activity was determined by using the agglutination test with 5% human blood cells. Human hepatocarcinoma cell HepG2 and the alloxan-induced diabetic mice model were used to assess hypoglycemic activity of Lectin in Trichosanthes kirilowi.
Results:
The agglutination indexes of lectins extraction buffer were 32; The cell and mice tests indicated that the lectins exhibited hypoglycemic activity in the 70% saturation.
Conclusion:
The optimum extraction technology is as follows: extraction with PBS, the material-water ratio is 1:30, the extraction time is 24 h, while the concentration of sodium chloride is 0 mol/L and pH is 7.2. Precipitate lectins by ammonium sulfate in the 70% saturation, centrifugal speed is 10 000 tracted from Trichosanthes kirilowi exposes proper hypoglycemic activity.
