Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 19, 2026

Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies
08:40

Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies

Published on: June 9, 2013

Photochemically induced ischemic stroke in rats.

Antje Schmidt1, Maike Hoppen1, Jan-Kolja Strecker1

  • 1Department of Neurology, University of Münster, Albert-Schweitzer-Campus 1, Gebäude A1, 48149, Münster, Germany.

Experimental & Translational Stroke Medicine
|August 11, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Depth of neutrophil mobilization stratifies survival in ST-elevation myocardial infarction.

Nature cardiovascular research·2026
Same author

Stroke Severity and Functional Benefit of Thrombectomy in Acute M2 Middle Cerebral Artery Occlusion: A Multicenter Cohort Study.

Neurology·2026
Same author

Ischemic Lesion Net Water Uptake Outperforms ASPECTS and CBF Less Than 30% in Predicting Futile Recanalization after Mechanical Thrombectomy.

Radiology·2026
Same author

Endovascular treatment for medium or distal vessel occlusion stroke (DISTAL): 12-month outcomes of a multicentre, open-label, randomised trial.

The Lancet. Neurology·2026
Same author

CD177 Expression on Neutrophils Predicts Ischemic Stroke Outcome in Humans.

Stroke·2026
Same author

How We Can Optimize Dysmenorrhea Treatment: Real-World Results from a Cross-Sectional, Multi-Center Study.

Geburtshilfe und Frauenheilkunde·2026

This study details a rat model for inducing photothrombotic stroke using Bengal Rose dye and laser illumination. This method creates localized cortical infarcts, enabling stroke research and therapy assessment.

Area of Science:

  • Neuroscience
  • Vascular Biology
  • Stroke Research

Background:

  • Photothrombosis, a model for ischemic stroke, was established in 1985.
  • This paper presents a refined protocol for inducing photothrombotic infarcts in rats.

Purpose of the Study:

  • To describe a reproducible protocol for inducing photothrombotic infarcts in rats.
  • To establish a model for studying ischemic stroke and evaluating neuroregenerative therapies.

Main Methods:

  • Intravenous administration of the photosensitive dye Bengal Rose.
  • Stereotactic positioning of a laser beam onto the intact rat skull.
  • Illumination induces localized thrombus formation in cortical microvessels.

Main Results:

More Related Videos

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke
08:07

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Published on: June 23, 2015

Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis
06:07

Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis

Published on: May 6, 2021

Related Experiment Videos

Last Updated: May 19, 2026

Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies
08:40

Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies

Published on: June 9, 2013

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke
08:07

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Published on: June 23, 2015

Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis
06:07

Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis

Published on: May 6, 2021

  • Photochemical activation of Bengal Rose leads to free radical formation.
  • Endothelial dysfunction and thrombus formation occur in illuminated cortical vessels.
  • This method reliably creates photothrombotic infarcts.

Conclusions:

  • Photochemically induced infarcts result in long-term sensorimotor deficits.
  • The model allows for long-term survival of subjects.
  • This rat model is suitable for assessing neuroregenerative therapies in chronic stroke studies.