Related Experiment Video
Updated: May 19, 2026
04:30
Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
Published on: June 19, 2018
Fibroblast growth factor 10 in human ovaries
Galia Oron1, Benjamin Fisch, Xiao Yun Zhang
1Infertility and IVF Unit, Hospital for Women, Rabin Medical Center, Petach Tikva 49100, Israel.
Reproductive Biomedicine Online
|August 11, 2012
Summary
Fibroblast growth factor 10 (FGF-10) is present in human ovarian follicles. This finding suggests FGF-10 may play a role in the development of preantral follicles.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Endocrinology
Background:
- The role of fibroblast growth factor 10 (FGF-10) in human ovarian cortical follicles remains uncharacterized.
- Understanding the expression of FGF-10 is crucial for comprehending early human oogenesis and follicle development.
Purpose of the Study:
- To investigate the expression of FGF-10 protein and mRNA in human preantral follicles across different life stages.
- To determine the potential contribution of FGF-10 to human preantral follicle development.
Main Methods:
- Immunohistochemistry was used to detect FGF-10 protein in ovarian tissue samples.
- Reverse-transcription PCR (RT-PCR) was employed to identify FGF-10 mRNA transcripts.
- Ovarian samples were analyzed from human fetuses (21-33 weeks gestation) and girls/women (5-39 years).
Main Results:
- FGF-10 protein was detected in oocytes (50%) and granulosa cells (30%) of fetal ovarian tissue.
- In girls and women, FGF-10 protein was consistently found in oocytes and granulosa cells of all samples.
- FGF-10 mRNA transcripts were identified in all tested fetal and adult ovarian samples.
Conclusions:
- FGF-10 is expressed at both protein and mRNA levels in human preantral follicles throughout development.
- The presence of FGF-10 suggests its involvement in the developmental processes of human preantral follicles.
- Further research into FGF-10 signaling pathways could elucidate its precise role in folliculogenesis.
Related Concept Videos
01:20Folliculogenesis
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
01:09Introduction to Fibroblasts
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
01:26Ovaries
The ovaries are roughly the size of almonds and measure approximately 2 to 3 centimeters in length. These paired structures are situated within the pelvic region and are anchored by the mesovarium—a peritoneal extension that also connects them to the wider structure of the broad ligament. The support system extends to the suspensory ligament, housing blood and lymphatic vessels. In addition, the ovarian ligament tethers the ovaries to the uterus.
On the ovarian surface, a layer of cuboidal...
On the ovarian surface, a layer of cuboidal...
01:24Gonadal and Placental Hormones
The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...
01:30Hormonal Control of the Ovarian Cycle
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...