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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: May 19, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Fluorescent signatures for variable DNA sequences.

John E Rice1, Arthur H Reis, Lisa M Rice

  • 1Department of Biology, Brandeis University, Waltham, MA 02454, USA.

Nucleic Acids Research
|August 11, 2012
PubMed
Summary
This summary is machine-generated.

A new polymerase chain reaction (PCR) method rapidly detects genetic variations using Lights-On/Lights-Off probes. This versatile technology aids in identifying diseases, pathogens, and organisms by analyzing DNA sequences efficiently.

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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Related Experiment Videos

Last Updated: May 19, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Genetic variations are crucial for identifying diseases, pathogens, and organisms.
  • Accurate and rapid detection of these variations is a cornerstone of molecular science and medicine.

Purpose of the Study:

  • To introduce a novel, highly informative closed-tube polymerase chain reaction (PCR) strategy.
  • To enable the analysis of both known and unknown DNA sequence variations.

Main Methods:

  • Utilizes LATE-PCR for efficient quantitative amplification of single-stranded DNA targets.
  • Employs sets of Lights-On/Lights-Off probes that hybridize over a broad temperature range.
  • Combines contiguous probe pairs and multiple colors for scanning and analyzing extended DNA sequences.

Main Results:

  • Generates sequence-specific fluorescent signatures from composite fluorescent contours.
  • Successfully characterized variant sequences in Mycobacterium tuberculosis, nematodes, and bacterial 16S ribosomal RNA genes.
  • Demonstrates versatility across diverse DNA targets.

Conclusions:

  • The developed Lights-On/Lights-Off probe PCR strategy offers a powerful tool for genetic variation analysis.
  • This technology is anticipated for widespread application in diagnostics, species identification, and fundamental research.
  • The method provides a versatile and efficient approach to sequence analysis.