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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: May 19, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Published on: September 3, 2013

Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array.

Siang Hooi Ling1, Yee Cheong Lam, Kerm Sin Chian

  • 1School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798.

Analytical Chemistry
|August 14, 2012
PubMed
Summary

This study introduces a novel dielectrophoretic cell separation technique using 3D electric fields. The method effectively separates heterogeneous cells based on their dielectrophoretic forces, achieving high purity and efficiency in continuous flow.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Separation Technologies

Background:

  • Dielectrophoresis (DEP) is a label-free cell manipulation technique.
  • Non-uniform electric fields are crucial for generating DEP forces.
  • Efficient cell separation is vital for various biological and medical applications.

Purpose of the Study:

  • To develop a dielectrophoretic cell separation method using three-dimensional (3D) nonuniform electric fields.
  • To achieve efficient separation of heterogeneous cells in a continuous flow system.
  • To demonstrate the method's effectiveness with biological cell mixtures.

Main Methods:

  • Generation of 3D nonuniform electric fields using a periodic array of asymmetric triangular bottom microelectrodes and a continuous top electrode.
  • Exploitation of differential positive dielectrophoretic forces acting on electrically polarized heterogeneous cells.
  • Separation of cells based on their trajectory deviations perpendicular to the fluid flow direction.

Main Results:

  • Achieved 87.3% pure live cells harvesting efficiency from a live/dead NIH-3T3 cells mixture.
  • Demonstrated separation of MG-63 cells from erythrocytes with 82.8% separation efficiency.
  • Confirmed that cells experiencing stronger DEP forces are streamed further from the main flow streamlines.

Conclusions:

  • The proposed 3D dielectrophoretic method offers a promising approach for continuous cell separation.
  • The microelectrode design effectively generates the necessary electric fields for differential cell manipulation.
  • This technology has potential applications in various fields requiring high-purity cell isolation.